Review



socs3b grna sequence  (Benchling Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Benchling Inc socs3b grna sequence
    (A) Fragments per kilobase million (FPKM) for all SOCS family members across all genotypes; significance represents the adjusted p value from DESeq differential analysis comparing all other samples to wild type. *p-adj < 0.05, **p-adj < 0.01, ***p-adj < 0.001, ****p-adj < 0.0001. (B) Schematic of <t>socs3b</t> gene structure and CRISPR target site. (C) Representative T7 assay results demonstrating high gRNA targeting efficiency in 4 independent samples of F0 animals compared to wild-type uninjected embryos; 10 embryos per sample (WT). (D) Quantification of SB+ cells in the CHT region of uninjected embryos (black bars) and F0 socs3b CRISPR injected embryos (red bars) at 3 dpf; n = 4–9/condition, *p < 0.05, **p < 0.01 by 2-way ANOVA with Tukey post hoc test. (E and F) Representative images of SB staining in 2-dpf uninjected or socs3b mutated tet2/3 DM embryos. Scale bars represent 500 μm. See also and .
    Socs3b Grna Sequence, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/socs3b grna sequence/product/Benchling Inc
    Average 90 stars, based on 1 article reviews
    socs3b grna sequence - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Tet Proteins Regulate Neutrophil Granulation in Zebrafish through Demethylation of socs3b mRNA"

    Article Title: Tet Proteins Regulate Neutrophil Granulation in Zebrafish through Demethylation of socs3b mRNA

    Journal: Cell reports

    doi: 10.1016/j.celrep.2020.108632

    (A) Fragments per kilobase million (FPKM) for all SOCS family members across all genotypes; significance represents the adjusted p value from DESeq differential analysis comparing all other samples to wild type. *p-adj < 0.05, **p-adj < 0.01, ***p-adj < 0.001, ****p-adj < 0.0001. (B) Schematic of socs3b gene structure and CRISPR target site. (C) Representative T7 assay results demonstrating high gRNA targeting efficiency in 4 independent samples of F0 animals compared to wild-type uninjected embryos; 10 embryos per sample (WT). (D) Quantification of SB+ cells in the CHT region of uninjected embryos (black bars) and F0 socs3b CRISPR injected embryos (red bars) at 3 dpf; n = 4–9/condition, *p < 0.05, **p < 0.01 by 2-way ANOVA with Tukey post hoc test. (E and F) Representative images of SB staining in 2-dpf uninjected or socs3b mutated tet2/3 DM embryos. Scale bars represent 500 μm. See also and .
    Figure Legend Snippet: (A) Fragments per kilobase million (FPKM) for all SOCS family members across all genotypes; significance represents the adjusted p value from DESeq differential analysis comparing all other samples to wild type. *p-adj < 0.05, **p-adj < 0.01, ***p-adj < 0.001, ****p-adj < 0.0001. (B) Schematic of socs3b gene structure and CRISPR target site. (C) Representative T7 assay results demonstrating high gRNA targeting efficiency in 4 independent samples of F0 animals compared to wild-type uninjected embryos; 10 embryos per sample (WT). (D) Quantification of SB+ cells in the CHT region of uninjected embryos (black bars) and F0 socs3b CRISPR injected embryos (red bars) at 3 dpf; n = 4–9/condition, *p < 0.05, **p < 0.01 by 2-way ANOVA with Tukey post hoc test. (E and F) Representative images of SB staining in 2-dpf uninjected or socs3b mutated tet2/3 DM embryos. Scale bars represent 500 μm. See also and .

    Techniques Used: CRISPR, Injection, Staining

    (A) RNA methylation status from RNA bisulfite-sequencing data for each CpG site in the socs3b locus for sorted wild-type or tet2/3 DM 2-dpf neutrophils; n = 10/condition. Red triangles indicate cytosines mutated in the differentially methylated cytosine (DMC) mutant mRNA used in the following experiment. (B) Relative levels of socs3b mRNA at 1–4 dpf assessed by qRT-PCR that measures levels of the injected wild-type or DMC mutant transcripts; n = 6 groups of 5 embryos per data point, *p < 0.05 by 2-way ANOVA with Tukey post hoc test. (C) Minimum free energy RNA structure of socs3b . DMCs are highlighted by triangles. Red triangles represent bases that were mutated in generating the DMC mutant socs3b mRNA and black triangles represent unedited bases. Inset: a close-up of an RNA arm with several DMCs closely packed together indicated by the red box in the full structure. See also and .
    Figure Legend Snippet: (A) RNA methylation status from RNA bisulfite-sequencing data for each CpG site in the socs3b locus for sorted wild-type or tet2/3 DM 2-dpf neutrophils; n = 10/condition. Red triangles indicate cytosines mutated in the differentially methylated cytosine (DMC) mutant mRNA used in the following experiment. (B) Relative levels of socs3b mRNA at 1–4 dpf assessed by qRT-PCR that measures levels of the injected wild-type or DMC mutant transcripts; n = 6 groups of 5 embryos per data point, *p < 0.05 by 2-way ANOVA with Tukey post hoc test. (C) Minimum free energy RNA structure of socs3b . DMCs are highlighted by triangles. Red triangles represent bases that were mutated in generating the DMC mutant socs3b mRNA and black triangles represent unedited bases. Inset: a close-up of an RNA arm with several DMCs closely packed together indicated by the red box in the full structure. See also and .

    Techniques Used: Methylation, Methylation Sequencing, Mutagenesis, Quantitative RT-PCR, Injection

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, SYBR Green Assay, Methylation, Phagocytosis Assay, CRISPR, Mutagenesis, Software



    Similar Products

    socs3b grna sequence  (Benchling Inc)
    90
    Benchling Inc socs3b grna sequence
    (A) Fragments per kilobase million (FPKM) for all SOCS family members across all genotypes; significance represents the adjusted p value from DESeq differential analysis comparing all other samples to wild type. *p-adj < 0.05, **p-adj < 0.01, ***p-adj < 0.001, ****p-adj < 0.0001. (B) Schematic of <t>socs3b</t> gene structure and CRISPR target site. (C) Representative T7 assay results demonstrating high gRNA targeting efficiency in 4 independent samples of F0 animals compared to wild-type uninjected embryos; 10 embryos per sample (WT). (D) Quantification of SB+ cells in the CHT region of uninjected embryos (black bars) and F0 socs3b CRISPR injected embryos (red bars) at 3 dpf; n = 4–9/condition, *p < 0.05, **p < 0.01 by 2-way ANOVA with Tukey post hoc test. (E and F) Representative images of SB staining in 2-dpf uninjected or socs3b mutated tet2/3 DM embryos. Scale bars represent 500 μm. See also and .
    Socs3b Grna Sequence, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/socs3b grna sequence/product/Benchling Inc
    Average 90 stars, based on 1 article reviews
    socs3b grna sequence - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Fragments per kilobase million (FPKM) for all SOCS family members across all genotypes; significance represents the adjusted p value from DESeq differential analysis comparing all other samples to wild type. *p-adj < 0.05, **p-adj < 0.01, ***p-adj < 0.001, ****p-adj < 0.0001. (B) Schematic of socs3b gene structure and CRISPR target site. (C) Representative T7 assay results demonstrating high gRNA targeting efficiency in 4 independent samples of F0 animals compared to wild-type uninjected embryos; 10 embryos per sample (WT). (D) Quantification of SB+ cells in the CHT region of uninjected embryos (black bars) and F0 socs3b CRISPR injected embryos (red bars) at 3 dpf; n = 4–9/condition, *p < 0.05, **p < 0.01 by 2-way ANOVA with Tukey post hoc test. (E and F) Representative images of SB staining in 2-dpf uninjected or socs3b mutated tet2/3 DM embryos. Scale bars represent 500 μm. See also and .

    Journal: Cell reports

    Article Title: Tet Proteins Regulate Neutrophil Granulation in Zebrafish through Demethylation of socs3b mRNA

    doi: 10.1016/j.celrep.2020.108632

    Figure Lengend Snippet: (A) Fragments per kilobase million (FPKM) for all SOCS family members across all genotypes; significance represents the adjusted p value from DESeq differential analysis comparing all other samples to wild type. *p-adj < 0.05, **p-adj < 0.01, ***p-adj < 0.001, ****p-adj < 0.0001. (B) Schematic of socs3b gene structure and CRISPR target site. (C) Representative T7 assay results demonstrating high gRNA targeting efficiency in 4 independent samples of F0 animals compared to wild-type uninjected embryos; 10 embryos per sample (WT). (D) Quantification of SB+ cells in the CHT region of uninjected embryos (black bars) and F0 socs3b CRISPR injected embryos (red bars) at 3 dpf; n = 4–9/condition, *p < 0.05, **p < 0.01 by 2-way ANOVA with Tukey post hoc test. (E and F) Representative images of SB staining in 2-dpf uninjected or socs3b mutated tet2/3 DM embryos. Scale bars represent 500 μm. See also and .

    Article Snippet: The Socs3b gRNA sequence was selected using the Benchling gRNA design software and crRNA was purchased from IDT.

    Techniques: CRISPR, Injection, Staining

    (A) RNA methylation status from RNA bisulfite-sequencing data for each CpG site in the socs3b locus for sorted wild-type or tet2/3 DM 2-dpf neutrophils; n = 10/condition. Red triangles indicate cytosines mutated in the differentially methylated cytosine (DMC) mutant mRNA used in the following experiment. (B) Relative levels of socs3b mRNA at 1–4 dpf assessed by qRT-PCR that measures levels of the injected wild-type or DMC mutant transcripts; n = 6 groups of 5 embryos per data point, *p < 0.05 by 2-way ANOVA with Tukey post hoc test. (C) Minimum free energy RNA structure of socs3b . DMCs are highlighted by triangles. Red triangles represent bases that were mutated in generating the DMC mutant socs3b mRNA and black triangles represent unedited bases. Inset: a close-up of an RNA arm with several DMCs closely packed together indicated by the red box in the full structure. See also and .

    Journal: Cell reports

    Article Title: Tet Proteins Regulate Neutrophil Granulation in Zebrafish through Demethylation of socs3b mRNA

    doi: 10.1016/j.celrep.2020.108632

    Figure Lengend Snippet: (A) RNA methylation status from RNA bisulfite-sequencing data for each CpG site in the socs3b locus for sorted wild-type or tet2/3 DM 2-dpf neutrophils; n = 10/condition. Red triangles indicate cytosines mutated in the differentially methylated cytosine (DMC) mutant mRNA used in the following experiment. (B) Relative levels of socs3b mRNA at 1–4 dpf assessed by qRT-PCR that measures levels of the injected wild-type or DMC mutant transcripts; n = 6 groups of 5 embryos per data point, *p < 0.05 by 2-way ANOVA with Tukey post hoc test. (C) Minimum free energy RNA structure of socs3b . DMCs are highlighted by triangles. Red triangles represent bases that were mutated in generating the DMC mutant socs3b mRNA and black triangles represent unedited bases. Inset: a close-up of an RNA arm with several DMCs closely packed together indicated by the red box in the full structure. See also and .

    Article Snippet: The Socs3b gRNA sequence was selected using the Benchling gRNA design software and crRNA was purchased from IDT.

    Techniques: Methylation, Methylation Sequencing, Mutagenesis, Quantitative RT-PCR, Injection

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Tet Proteins Regulate Neutrophil Granulation in Zebrafish through Demethylation of socs3b mRNA

    doi: 10.1016/j.celrep.2020.108632

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: The Socs3b gRNA sequence was selected using the Benchling gRNA design software and crRNA was purchased from IDT.

    Techniques: Recombinant, SYBR Green Assay, Methylation, Phagocytosis Assay, CRISPR, Mutagenesis, Software